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【Proteintech】螢光標記 - FlexAble Antibody Labeling Kits

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【Proteintech】螢光標記 - FlexAble Antibody Labeling Kits

 【Proteintech】FlexAble Antibody Labeling Kits

 

Proteintech_FlexAble antibody labeling kits 是一種新穎的抗體標記試劑盒並以高親和力、非共價的方式將一級抗體進行螢光標記。

FlexAble Antibody Labeling Kits 產品資訊

 

 

Conjugate Size Compatible with
CoraLite Plus 405 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1,Mouse IgG2a
FITC Plus 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1, Mouse IgG2a
CoraLite Plus 488 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b
CoraLite Plus 555 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b
CoraLite Plus 647 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1, Mouse IgG2a, Mouse IgG2b
CoraLite Plus 750 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1, Mouse IgG2a

 

CoraLite Plus spectra

 

Proteintech's CoraLite Plus excitation and emission

 

CoraLite Plus dyes 的亮度與 Alexa Fluor® dyes 相當。由於重疊熒光光譜最小，可同時用於共定位研究 （co-localization studies）
 

FlexAble 的 CoraLite Plus 系列實現更快、更簡單的多重檢測。未來還會新增更多 dyes/specificities ~ This is just the start!

 

應用項目 Immunofluorescence (IF), Western blotting (WB), Flow cytometry (FC)

優勢項目

  • 不限任何品牌的一級抗體皆能使用本產品 ( 無須抗體純化 / 緩衝液置換 )

  • 快速兩步驟且僅須 10 分鐘完成一級抗體標記 (支持多重染色)

  • 排除物種交叉反應所造成的背景誤差與抗體配置困擾

  • 適用於任何抗體濃度和配方 ( FlexAble 有利於低濃度抗體標記 )

  • 高濃度抗體無須稀釋，低濃度抗體不須濃縮 (可低至 0.06 mg/mL )

  • 不須置換抗體緩衝液
    Carriers ( 載體系列抗體 ): BSA, glycerol ( 50% )
    Amine-containing buffers ( 緩衝溶液 ): Tris
    Preservatives ( 防腐劑 ): sodium azide ( NaN₃, 疊氮化鈉 )

  • 1 組 labeling kit (50 reactions ) 可標記多達 50 種不同的抗體，無需特殊設備
     

Antibody labeling with FlexAble 原理

FlexLinker 以高親和力、非共價的方式進行抗體標記 (Fc region of antibody)，一旦結合就不會從抗體中釋放出來，而未與抗體結和的 FlexLinker 將被 FlexQuencher 中和 ( Neutralization )。

Proteintech 一鍵優化實驗流程 ( 標記一抗 0.5 µg )

 

 

It's easy as 1, 2, 3
3步驟, 10 分鐘, 完成標記

 

Step-by-step workflow of Proteintech's FlexAble antibody labeling kit shows 30 seconds hands-on time and ready-to-use in less than 10 minutes

* 根據一抗標記量，適當比例調整試劑體積

想了解更多 點入觀看影片 Watch how FlexAble works

 

Multiplexing (可應用於多重染色 排除物種交叉反應 )

同物種的一級抗體，須分別標記。標記完成，可將其合併應用

FlexAble provides the easiest way to multiplex antibodies from the same species and isotype

不同物種的一級抗體可以在同一試管中混合，使用相對應物種的 FlexLinker 同時進行標記

Primary antibodies of different species can be labeled simultaneously in the same tube with FlexAble antibody labeling kits.

應用範例

FlexAble Antibody Labeling Kits_Immunofluorescence 

HeLa cells were stained with anti-TOM70 (14528-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green), anti-GNL3 (67169–1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Cell nuclei are in cyan.

 

HeLa cells were stained with anti-Lamin labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue).

 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

 

FlexAble Antibody Labeling Kits_ Flow cytometry

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble FITC Plus Kit (KFA048).

 

1X10^6 Jurkat cells were intracellularly stained with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble FITC Plus Kit (KFA008, red) or with isotype control antibody labeled with FlexAble FITC Plus Kit (KFA008, cyan).​

 

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

 

FlexAble Antibody Labeling Kits_Western blotting

Western Blot analysis of HeLa and HepG2 cell lysates with anti-HSP90 antibody (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP antibody (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM antibody (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).​

 

WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

 

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

 

 

更多免疫螢光染色技術探討請觀看 ABreal biotech 倍思特生物科技 YouTube 頻道
 

【倍思特研討會】Proteintech 光影交錯: 免疫螢光染色的技術與創新
Innovations and Advances in Immunofluorescence 
 

Dr. Klaus Herick 將帶領您探索免疫螢光顯微鏡試劑的最新創新和進步

  • 創新多重染色技術   
    抗體標記技術的多重染色 (multiplexing)：不僅擴展了傳統的一抗和螢光二抗標記，還能同時使用多種不同的抗體進行螢光標記，進一步擴大了免疫螢光檢測的應用範圍。
     
  • 超越交叉反應與雜訊 
    在免疫螢光檢測中，Dr. Klaus Herick 將介紹最新的螢光染料，這些染料具有更高的亮度和光穩定性，不僅可以提高檢測靈敏度和準確性，還可以在多樣的樣本類型和實驗條件下進行應用，為免疫螢光檢測技術的發展帶來更多的可能性。
     
  • 微小探針、大視野 突破分辨率極限 
    探索羊駝衍生納米抗體 (Nanobodies) 的優勢和應用: 討論納米抗體用於檢測一抗和其他主要表位 (epitopes) 的靈活性，並分享使用納米抗體進行組織和器官/動物成像的實例應用。

​
演講者: Dr. Klaus Herick (APAC Commercial Lead, Proteintech Group Inc)

 

相關資訊：
 

  1. 【ABreal】【倍思特研討會】Proteintech光影交錯：免疫螢光染色的技術與創新
     
  2. 【Proteintech】FlexAble Antibody Labeling Kits
     
  3. 【Proteintech】 Directly conjugated CoraLite antibodies for Immunofluorescence
     
  4. 【Proteintech】 Nano-Secondary reagents
     
  5. 【Proteintech】 Immunofluorescence

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