HumanKine® recombinant human TGF beta 1 protein(Cat No. HZ-1011)
HumanKine® recombinant human TGF beta 1 protein
(Cat No. HZ-1011)
HEK293 expressed, Endotoxin-free, Animal-component free
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Synonyms：
CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1
Technical Specifications：
| GeneID | 7040 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.01-0.17 ng/mL |
| Specific Activity | minimally 1.00 x 10^7 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P01137 |
| Molecular Mass | 13 kDa reduced, 25 kDa non-reduced, homodimer, non-glycosylated |
| Formulation | 50 mM NaOAc pH 3.7, See Certificate of Analysis for details |
| Species Reactivity | Human, Mouse |
Validation Data Gallery：
 |
Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue. |
%20inhibits%20IL-4%20induced%20proliferation%20of%20the%20HT-2%20mouse%20cell%20line.png) |
Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL. |
%20residing%20within%20an%20in%20vivo%20brain-like%20organoid%20environment%20(blue)%20derived%20using%20HumanKine%20growth%20factors%20(TGF%20beta%201-%20HZ-1011%2C%20BMP4-%20HZ-1045%2C%20and%20Thrombopoietin%20HZ-1248).jpg) |
Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems) |
Stability and Reconstitution：
| Stability & Storage | Product Form | Temperature Conditions | Storage Time | ||
| Lyophilized | -20°C to -80°C | Until Expiry Date | |||
| Room Temperature | 2 weeks | ||||
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |||
| 4°C | 1 week | ||||
| Avoid repeated freeze-thaw cycles. | |||||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. | ||||
Whitepapers
Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells
Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells
Efficient Induction of Th17 Cells with HumanKine® TGFβ1
Background Information：
TGFB，又稱LAP和TGFB1，是一種多功能細胞激素，可調控細胞增生、分化和凋亡。 TGF與TGFA協同作用，誘導細胞轉化的過程。它也作為一種抑制性自分泌生長因子發揮作用，在TGFB活化和訊號傳導失調的情況下，可能導致細胞凋亡。有許多細胞能夠合成TGFB且具有其特異性受體，使得TGFB對許多其他生長因子具有正向和負向調控作用。它在骨重塑/重建中發揮重要作用，是成骨細胞骨形成的強效刺激因子（PMID：11586292）。
Datasheet：
https://www.ptglab.com/products/pictures/pdf/HZ-1011.pdf
資料來源：
Proteintech：HumanKine® recombinant human TGF beta 1 protein(Cat No. HZ-1011)
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