瀏覽數：277

HumanKine® recombinant human TGF beta 1 protein- GMP grade(Cat No. HZ-1011-GMP)

HumanKine® recombinant human TGF beta 1 protein
- GMP grade
(Cat No. HZ-1011)

HEK293 expressed, Endotoxin-free, Animal-component free

Size：10 μg, 100 μg, 1000 μg, Custom

Synonyms：

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Technical Specifications：

GeneID	7040
Species	Human
Expression	HEK293
Activity(Primary)	0.01-0.17 ng/mL
Specific Activity	minimally 1.00 x 10^7 IU/mg, typically 1.24 x 107 IU/mg
Purity	>95%
Endotoxin	<0.1 EU/µg
Accession Number	P01137
Molecular Mass	13 kDa reduced, 25 kDa non-reduced, homodimer, non-glycosylated
Formulation	50 mM NaOAc pH 3.7, See Certificate of Analysis for details
Species Reactivity	Human, Mouse

Validation Data Gallery：

Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

Stability and Reconstitution：

Stability & Storage	Product Form	Temperature Conditions	Storage Time
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells
Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells
Efficient Induction of Th17 Cells with HumanKine® TGFβ1

GMP Quality Policies：

HumanKine® GMP (Good Manufacturing Practice) 重組蛋白的生產和驗證均符合 ISO 13485 品質管理體系，並符合 GMP 標準。
我們的 GMP 重組蛋白不含動物源成分 (animal component free, ACF)、不含異種成分 (xeno free, XF) 且不含標籤 (tag free, TF)。
按此了解更多相關資訊。

Background Information：

TGFB，又稱LAP和TGFB1，是一種多功能細胞激素，可調控細胞增生、分化和凋亡。 TGF與TGFA協同作用，誘導細胞轉化的過程。它也作為一種抑制性自分泌生長因子發揮作用，在TGFB活化和訊號傳導失調的情況下，可能導致細胞凋亡。有許多細胞能夠合成TGFB且具有其特異性受體，使得TGFB對許多其他生長因子具有正向和負向調控作用。它在骨重塑/重建中發揮重要作用，是成骨細胞骨形成的強效刺激因子（PMID：11586292）。

Datasheet：

https://www.ptglab.com/products/pictures/pdf/HZ-1011-GMP.pdf

資料來源：

Proteintech：HumanKine® recombinant human TGF beta 1 protein- GMP grade(Cat No. HZ-1011-GMP)

產品資訊